Thompson, A. W., Vanwalleghem, G. C., Heap, L. A., & Scott, E. K. (2016). Functional profiles of visual-, auditory-, and water flow-responsive neurons in the zebrafish tectum. Current Biology, 26(6), 743-754.
Ca imaging with selective plane microscopy. Good refs on cell ensembles. They find ensembles that selectively encode stimulus features. PCA+promax, then clustering to identify them. Classification depends on threshold and manual elimination of "artifactual clusters", so not too objective. Inputs for each cluster tend to be in same laminae (layers). For moving bars, not much correlation with position, suggesting that it's not retinotopy, but true directionality. Most cells are unimodal (only 1-3% belong to two clusters).
Implications: Consider that there are many cells with different sensory / multisensory properties, so it is only to be expected that they would be tuned differently, and possibly via different mechanisms. Especially if they are starting to segregate into layers, as this would imply different distances from the soma to the dendrites and (potentially) the axon initial segment.
Ca imaging with selective plane microscopy. Good refs on cell ensembles. They find ensembles that selectively encode stimulus features. PCA+promax, then clustering to identify them. Classification depends on threshold and manual elimination of "artifactual clusters", so not too objective. Inputs for each cluster tend to be in same laminae (layers). For moving bars, not much correlation with position, suggesting that it's not retinotopy, but true directionality. Most cells are unimodal (only 1-3% belong to two clusters).
Implications: Consider that there are many cells with different sensory / multisensory properties, so it is only to be expected that they would be tuned differently, and possibly via different mechanisms. Especially if they are starting to segregate into layers, as this would imply different distances from the soma to the dendrites and (potentially) the axon initial segment.
