Helmbrecht 2018

Helmbrecht, T. O., dal Maschio, M., Donovan, J. C., Koutsouli, S., & Baier, H. (2018). Topography of a Visuomotor Transformation. Neuron.

In ZF, two distinct pathways from OT to HB: one for prey, another for avoidance. Rev: damage to OT prevents orienting towards prey. Optogenetics: stimulate diff neurons in the OT, detect motor outputs. Clustering: 2 distinct behav: escapes and approaches. Moreover, retinotopic position and eye position are integrated, to calculate the maneuver. Combined with whole-brain gcamp img, observed targeted HB neurons activated. Then traced individ neurons and classified into 8 classes, based on projection targets, including ipsi- and contra- to HB, several distinct type of commissural, etc. Up-down retinotopy is less precisely encoded than LR (aka rostro-caudal).

Haas 2001

Haas, K., Sin, W. C., Javaherian, A., Li, Z., & Cline, H. T. (2001). Single-cell electroporationfor gene transfer in vivo. Neuron, 29(3), 583-591.

Among other things, describes that each cell in XL OT is about 20 um in diameter.

Williamson 2018

Williamson, W. R., Peek, M. Y., Breads, P., Coop, B., & Card, G. M. (2018). Tools for Rapid High-Resolution Behavioral Phenotyping of Automatically Isolated Drosophila. Cell Reports, 25(6), 1636-1649.

[Williamson 2018] Describes some outrageously sci-fi equipment for automated tracking of fruitflies, one by one, with a dispensor, an optogenetic setup, and virtual reality dome that employes a conical mirror! Very inspiring, and also a great video-dictionary of elementary behaviors (fixed action patterns?) towards the end.

Abbas 2017

Abbas, F., Triplett, M. A., Goodhill, G. J., & Meyer, M. P. (2017). A Three-Layer Network Model of Direction Selective Circuits in the Optic Tectum. Frontiers in neural circuits, 11, 88.

[Abbas 2017] Define 3 layers as: axons of RGCs, supervisial inhibitory interneurons (SIN), and principal or paraventricular neurons (PVN). ZF larvae. Claim that at first 2 levels there are only 3 principal directions (a trifoil, equally split), but they get recalculated into 4 at last layer (rostro-caudal is absent in the RGCs input, but is present in OT). In intro: there seems to be a controversy in ZF, about how exactly inhibition is tuned in direction-selective circuits in the OT, and whether it is sharpening, or defining the responses. Model: just 10 neurons, as each one represents an entire population; 3 layers, ReLU units, diff equations (not too discrete). Custom set input sensitivity curves and tuned connectivity scheme, reproduces tuning from Ca img experiments